About the Lab
The Jaiswal Laboratory is at the Children’s National Research Institute and the department of Genomics and Precision Medicine at the George Washington University School of Medicine. Work in our laboratory examines the cellular machinery that regulates the inter- and intracellular trafficking, and how defects in this are caused by genetic diseases, injury or infection. We study injury and repair processes using skeletal muscle – a tissue that has the unique ability to not only fully recover from routine mechanical injuries but whose functionality is enhanced by exercise-induced injuries.
Studies from our lab have revealed unexpected molecular and cellular mechanisms that enable repair of muscle cell and tissue that are conserved among other cell types and across multiple diseases. By using a combination of disease models and single cell approaches, we have examined the cellular and molecular deficits that impair muscle repair, and developed approaches to tackle these deficits. Our studies also examine how viruses subvert the host cell’s trafficking processes and what this host-pathogen arms race teach us about the biology of healthy cell and controlling viral infections. Our ongoing studies continue to aim to understand the basic biology of cellular trafficking and apply this knowledge to develop new therapies, some of which have advanced to pre-clinical and clinical stages.
Mitochondrial fragmentation enables localized signaling required for cell repairHorn A, Raavicharla S, Shah S, Cox D, Jaiswal JK J Cell Biology 219(5):e201909154. doi: 10.1083/jcb.201909154 (2020)
Fibroadipogenic progenitors are responsible for muscle loss in limb girdle muscular dystrophy 2BHogarth MW, Defour A, Lazarski C, Gallardo E, Diaz Manera J, Partridge TA, Nagaraju K, Jaiswal JK Nature Communications 10(1):2430. doi: 10.1038/s41467-019-10438-z (2019)
Membrane stabilization by modified steroid offers a potential therapy for muscular dystrophy due to dysferlin deficitSreetama SC, Chandra G, Van der Meulen JH, Ahmad MM, Suzuki P, Bhuvanendran S, Nagaraju K, Hoffman EP, Jaiswal JK Molecular Therapy 26(9):2231-2242. doi: 10.1016/j.ymthe.2018.07.021 September (2018)
Mitochondrial redox signaling enables repair of injured skeletal muscle cellsHorn A, Van der Meulen JH, Defour A, Hogarth M, Sreetama SC, Reed A, Scheffer L, Chandel NS, Jaiswal JK Science Signaling 10(495):eaaj1978. doi: 10.1126/scisignal.aaj1978 (2017)
Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repairScheffer LL, Sreetama SC, Sharma N, Medikayala S, Brown KJ, Defour A, Jaiswal JK Nature Communications 5:5646. doi: 10.1038/ncomms6646 (2014)
Gene therapy offers potential long-term treatment for limb-girdle muscular dystrophy 2BJanuary 04, 2022
A new pre-clinical gene therapy for limb-girdle muscular dystrophy 2B addresses the primary cellular deficit associated with the disease.Read More